Constitutive expression of Egr-1 is reduced in FA-A cells. Total RNA and cell lysates were purified from control and FA-A lymphoblast cell lines and analyzed for Egr-1 mRNA and protein levels by semiquantitative RT-PCR (A) and Western blot (B), respectively. GAPDH mRNA was used as an amplification control. The levels of β-tubulin were analyzed to assure equal loading. (C) Control and FA-A cells were transfected with a 688-bp fragment of the Egr-1 promoter in the presence of a β-galactosidase reporter vector, and the Egr-1 promoter-dependent transcription was determined. Units of luciferase activity were normalized based on values of β-gal activity to control for transfection efficiency. Histograms represent the means ± SD of triplicate analyses.