Imatinib mesylate inhibits activation of

DCs. DCs generated from CD34⁺ cells with GM-CSF, TNF-α, IL-4, and FLT3L in the presence of 3 μM imatinib mesylate were incubated with soluble CD40L and IFN-γ as a maturation stimulus 24 hours before harvesting the cells. The effect of imatinib mesylate on DC phenotype was analyzed by FACS. The surface expression is indicated as mean fluorescence intensity.