Imatinib mesylate modulates mRNA level of MIP-1α, ELC, and CCR7 in DCs. RNA was extracted from DCs generated from mobilized human CD34⁺ PBPCs that were cultured in the presence of GM-CSF, TNF-α, IL-4, and FLT3L with or without imatinib mesylate (3 μM and 5 μM). For activation of DCs, cells were incubated with soluble CD40L and IFN-γ 24 hours before harvesting the cells. Transcription of MIP-1α, ELC, CCR7, and RelB was analyzed by RT-PCR. Amplification of β₂microglobulin (β2m) served as internal control to ensure the use of equal amounts of cDNA.