Clonal analysis of expanded CD8⁺T cells from AIM blood and tonsil samples. (A) Representative dot plot of CD8 versus tetramer (RAK) staining in matched blood and tonsil samples. Heteroduplex analysis was used to assess the clonality of purified CD8⁺ T cells from matched blood and tonsil samples taken from 3 AIM patients. PCR reactions were performed for 23 different Vβ families in the blood and tonsils of each patient. (B) Data from a representative Vβ family showing heteroduplex bands shared between CD8⁺ T cell blood and tonsils, as indicated by solid arrows, are shown. Dashed arrow indicates the homoduplex band.