Engraftment of lentivirus-transduced EGFP/EYFP-expressing baboon CD34-enriched cells in NOD/SCID mice. Displayed are representative flow cytometry data from mice that received transplants in parallel with the autologous baboon transplants. Forward-scatter/side-scatter (FSC/SSC) gating to exclude debris and propidium iodide (PI) gating to exclude dead cells was applied to all plots. (A) Isotype control staining of a mouse that received a transplant of EGFP-expressing lentivirustransduced A00074 cells (MOI 10) showing GFP-positive events in the lower right quadrant. (B) CD11a-PE staining of the same mouse in panel A showing engraftment of transduced and nontransduced baboon cells. (C) CD11a-PE staining of a mouse that received a transplant of EYFP-expressing lentivirus-transduced A00083 cells (all growth factors). (D) CD11a-PE staining of a mouse that received a transplant of mock-transduced A00074 cells. Given are the percent engraftment (ie, % CD11a⁺) and the percent of CD11a⁺ cells expressing EGFP/EYFP.