Detection of EGFP/EYFP-specific cytotoxic T-lymphocyte responses. Percent specific lysis of mock-transduced (▪) or EYFP-transduced (□) autologous targets by in vitro–stimulated PBMCs from M00067 (A), K99307 (B), A00083 (C), M99149 (D), EGFP/EYFP-tolerant transplanted control animal M99267 (E), and naive control animal T99151 (F). Assays were done in triplicate at 3 effector-to-target (E/T) ratios as indicated.