Figure 5.
Morphologic changes, apoptosis, and cell death induced in CAG myeloma cells (TRF, 2.7 kb) after 7 days of in vitro GRN163 (10 μM) treatment. CAG myeloma cells (1 × 105) were prepared in 6-well plates with PBS, GRN163 (1-10 μM), or a mismatch oligonucleotide control 227 (10 μM). Cells were harvested, counted, and replated in the presence of fresh drug on day 4. On day 7 of treatment, cells were collected and (A) viewed under × 60 light microscopy for morphologic changes. (B) Dual-color staining of cells for annexin/PI uptake indicative of cell apoptosis and necrosis was also performed.
