Southern blot analysis for provirus integration sites within the femur, BM, and spleen of NOD/SCID mice engrafted with noncultured and cultured lentivector-transduced cells. Southern blot analysis of the clonal kinetics in mice engrafted with noncultured (A) and cultured (B) cells. Lanes labeled Fem were loaded with DNA from a femur aspirate at the indicated time points, whereas BM and spleen (Spl) lanes contained DNA from these tissues at 12 weeks. Each unique band has been numbered. The presence of the same-sized bands at multiple time points and different hematopoietic tissues is denoted by rectangles. (C) Comparison of the clonal composition within the BM of mice given transplants of day-1 and day-4 cells from the same CB sample and analyzed at 12 weeks. (D) Total number of clones present in femur, BM, and spleen at 12 weeks after transplantation. Each bar represents the mean data (± SE) from the mice (n = 9 for day 1 and n = 6 for day 4) where the number of clones could be determined in all hematopoietic tissues.