Reduction of proinflammatory cytokines by adenoviral BPI gene transfer during endotoxemia. Mice injected intraperitoneally with either AdhBPI or AdDl70.3 (5 × 10⁸ pfu) were challenged with LPS (0.8 mg/kg). Serum samples were taken at 1, 1.5, and 2.5 hours after LPS challenge and measured by ELISA for TNF-α (A) and MIP-2 (B). Results are expressed as means ± SEM from 5 to 11 mice/group. The difference between AdhBPI and AdDl70.3 control is statistically significant (P ≤ .009, P ≤ .02, and P ≤ .002 for 1, 1.5, and 2.5 hours, respectively, for TNF-α; P ≤ .01, P ≤ .000 005, and P ≤ .0006 for 1, 1.5, and 2.5 hours, respectively, for MIP-2).