mtDNA heterogeneity in individual CD34⁺ clones. (A) Sequence chromatogram with mixed nucleotide signal (G/A) at nucleotide position 200 in HV2 from 1 CD34⁺ clone of BM donor no. 6 (left), and then was clearly divided into wild type (center) and mutation (200A>G/A) (right) after TA cloning. (B) Poly C length heteroplasmy between nucleotide position 303 and 315 from one of the CD34⁺ clones of BM donor no. 3 (left). Reverse-sequence analysis after TA cloning revealed poly C length heteroplasmy of 8CT6C (center) and 9CT6C (right).