Expression of the fractalkine receptor (CX₃CR1) on platelets demonstrated by Western blot (human platelets) or flow cytometry analysis (rat platelets). (A) Western blot demonstrating CX₃CR1 expression on human washed platelets compared with peripheral blood mononuclear cells (PBMCs) and human spleen lysate as a positive control for CX₃CR1. (B) The expression of CX₃CR1 was similar in platelets from healthy Wistar-Kyoto rats (WKY) in the absence or presence of stimulation with adenosine diphosphate (ADP). The flow cytometry data are expressed as mean fluorescence ± SEM from 6 separate experiments. (C) A typical flow cytometry histogram with background (fine) and CX₃CR1 expression (bold) on platelets from Wistar-Kyoto rats.