American Society of Hematology

Figure 4.

$Figure 4. Effect of fractalkine on platelet adhesion to fibrinogen. Firm adhesion of washed human platelets to fibrinogen was assessed in a flow chamber model under high (A) and low (B) shear following stimulation with rhCX3C (1 μg/mL) or ADP (5 μM) in the presence and absence of apyrase (7.5 U/mL). The proadhesive effect was blocked by preincubation with an antagonizing antibody against fractalkine (AF537; 100 g/mL; 30 min) as well as by disruption of CX3CR1-mediated signaling with PTX (0.4 nM; 45 min). Effect of inhibition of glycoprotein IIb/IIIa by preincubation with the monoclonal antibody c7E3 (4 μg/mL). Data are expressed as the ratio of adhering platelets to the control group (□) ± SEM from at least 5 separate experiments, *P < .05 versus control (CO); **P < .01 versus Co; #P < .05 versus stimulation; ##P < .01 versus stimulation.$

Effect of fractalkine on platelet adhesion to fibrinogen. Firm adhesion of washed human platelets to fibrinogen was assessed in a flow chamber model under high (A) and low (B) shear following stimulation with rhCX₃C (1 μg/mL) or ADP (5 μM) in the presence and absence of apyrase (7.5 U/mL). The proadhesive effect was blocked by preincubation with an antagonizing antibody against fractalkine (AF537; 100 g/mL; 30 min) as well as by disruption of CX₃CR1-mediated signaling with PTX (0.4 nM; 45 min). Effect of inhibition of glycoprotein IIb/IIIa by preincubation with the monoclonal antibody c7E3 (4 μg/mL). Data are expressed as the ratio of adhering platelets to the control group (□) ± SEM from at least 5 separate experiments, *P < .05 versus control (CO); **P < .01 versus Co; #P < .05 versus stimulation; ##P < .01 versus stimulation.

This Feature Is Available To Subscribers Only