Figure 4.
Effect of urea and ion strength on cleavage. GST-VWF73-H was incubated with normal plasma for the indicated time in reaction buffer (5 mM Tris-HCl, 10 mM BaCl2, pH 8.0) (lanes 1-3) or in the same buffer supplemented with either 1.5 M urea (lanes 4-6) or 150 mM NaCl (lanes 7-9). The substrate and product bands are shown by an arrow and an arrowhead, respectively.
