Figure 2.
Effect of imatinib mesylate on FIP1L1-PDGFR fusion. Nested RT-PCR results for the FIP1L1/PDGFR fusion gene in PBMC RNA from 6 patients before (lanes a) and after (lanes b) treatment with imatinib mesylate. The lower panel shows the results of RT-PCR for the GAPDH gene as a control for amount of RNA loaded for each patient sample. Various size bands are observed in different cases due to different breakpoints in the FIP1L1 gene as shown previously.1 The different bands in each lane represent splice variants. The FIP1L1-PDGFR fusion became undetectable in 5 of 6 patients after imatinib mesylate therapy.
