Increased cell-surface expression of G-CSF-R/EGFP on cells in hypertonic sucrose or methyl-β-cyclodextrin. Stably transfected 3T3 cells were plated on glass coverslips and either left untreated (left panel) or incubated in 0.45 M sucrose (middle panel) or 5 mM methyl-β-cyclodextrin (mβCD, right panel) for 3 hours before fixation and examination by confocal microscopy. Confocal micrographs are representative of the distribution of wt G-CSF-R/EGFP in the entire cell population. Scale bars are 10 μm.