Increased adhesion of murine HS/HE RBCs to thrombospondin (TSP) and laminin (LM). Washed RBCs from control (WBB6F₁-+/+ and -sph/+; CcS3/Dem-+/+ and -sph^Dem/+), elevated reticulocyte control (WBB6F₁ Hi-ret), and mutant (severe HS: WBB6F₁-sph/sph, -sph^2BC/sph^2BC, and -sph^J/sph^J; severe HE: CcS3/Dem-sph^Dem/sph^Dem) mice were perfused through flow chambers previously coated (2 μg/cm²) with human TSP (A) or human LM (B) at a wall shear stress of 1 dyne/cm². Adherent RBCs per unit area were counted by direct microscopic visualization as described in “Materials and methods.” Group/genotype is listed on x-axis just below bars; numbers of measurements per group are indicated in parentheses below genotype/group. Strain background for each group is indicated between horizontal arrows. Adherent RBCs/mm² are depicted as mean ± SEM. *P < .001 versus +/+ values.