Impaired sialylation of NKp46D2-Ig in CHO cells significantly reduces HA binding. (A) 721.221 cells were infected with SV, human influenza A virus H1N1 (New Caledonia), and H3N2 (Sydney). Infected cells were stained with NKp46D2-Ig produced in either COS (▪) or CHO (□) cells followed by PE-conjugated goat antihuman Fc. Figure shows one representative experiment of 3 performed. (B) ELISA plates were coated with various concentrations of HA rosettes ranging from 0.075 μg/well to 0.3 μg/well as indicated, followed by incubation with 0.2 μg of the relevant fusion protein. Bound proteins were detected using AP-conjugated second mAb. Shown is one representative experiment of 2 performed.