Analysis of platelet proteins, albumin, IgG, and fibrinogen. (A) Platelet lysates were subjected to SDS-PAGE. Gelcode blue-stained gel showing a decrease in an approximate 66-kDa band in the patient (P) but not the family members (B, S) or control subjects (N). M represents molecular weight markers. (B) Immunoblot analyses of albumin, IgG, and fibrinogen in platelet lysates. In the first (50% B) and the last (50% N) lanes, half of the corresponding sample (B, N) was applied, to assess the sensitivity of the analysis. (C) Immunoblot analysis of albumin in plasma. The albumin in patient plasma sample was comparable to that of 3 control subjects (N).