NOD/scid lentiviral transgenesis and hGM-CSF expression. (A) Schematic diagram of the prrl CMV hGM-CSF lentiviral vector. (B) Proviral transgene insertion in F0 mice (a-r) generated by subzonal hGM-CSF–lentivirus vector microinjection of single-cell embryos. A Southern blot analysis of BamHI-digested tail genomic DNA probed with a 270-bp fragment corresponding to a wpre gene present in the lentiviral vector is shown. Each band represents a proviral insertion. (C) Germ line transmission and allele segregation of the proviral transgene. A Southern blot analysis of genomic DNA of founder (Z) and F1 offspring (z.1 to z.11) resulting from the cross with a wild-type NOD/scid mouse is shown. (D) hGM-CSF expression in serum of hGM-CSF transgenic (tg+), nontransgenic (tg-), and wild-type (wt) NOD/scid mice. Each circle indicates an individual mouse and each horizontal bar shows an arithmetical average.
