In vitro Vegfr2-luc expression and regulation. The p Vegfr2-luc transgene construct was transiently transfected into cells in culture, and transfection efficiency was normalized by cotransfection with pRL-TK renilla luciferase vector. (A) Comparison of luciferase expression in BAEC, LL/2, MLTC-1, T241, and HepG2 cells. (B) Transiently transfected BAECs were treated with angiogenesis inhibitor mithramycin (Mith), 2-methoxyestradiol (ME), or fumagillin (FU), as described in “Materials and methods.” C indicates control (C) Effect of cell proliferation on luciferase expression in BAEC and HeLa cells. Results are expressed as a percentage of luciferase activity relative to appropriate control. RLU indicates relative light unit. These experiments were repeated twice. Data are presented as mean ± SE.