Figure 6.
Figure 6. Histologic assessment of wound healing and response to DEX. Paraformaldehyde-fixed and stained sections are shown from the skin of nonwounded mice and from mouse wounds isolated 7 days after wounding in female Vegfr2-luc mice treated with saline or DEX (n = 4 per group). Sections were taken vertically through the granulated tissue extending from the surface into the adipose and skeletal muscle. In the sections shown, the surface of the skin or granulated tissue is at the top of the image, and in some images the deeper adipose tissue and skeletal muscle are readily visible. (A) Blood vessels (red arrows) were visualized by staining with an anti-CD31 antibody. The granulation tissue of saline-treated wounds showed blood vessels that were of relatively small caliber. In DEX-treated wounds, the blood vessels in the granulated tissue were of larger caliber. Dermal blood vessels in the normal nonwounded skin tissue were substantially less dense. Original magnification, × 200. (B) Infiltration of leukocytes was visualized by staining with anti-CD11b antibody. There were few scattered leukocytes (red arrows) in normal nonwounded skin. Blue arrow points to hair follicles in normal skin. Saline-treated wounds had significant leukocyte infiltration that appeared to consist predominantly of macrophages. DEX-treated wounds had markedly less macrophage infiltration. Original magnification, × 200. (C) The presence of the VEGFR2 receptor in the tissue was assessed by immunohistochemical staining with an anti-VEGFR2 antibody. Compared with normal nonwounded skin tissue, saline-treated wounds showed increased staining in endothelial cells and in stromal cells. Staining intensity was higher in cells closest to the lesioned epithelium of the wound, with less staining in deeper tissue. Red arrows indicate blood vessels. DEX-treated wounds had less VEGFR2 staining. Original magnification, × 100. (D) At a higher magnification, the specific cell types that positively stained for VEGFR2 were more apparent. Red arrows indicate blood vessel endothelial cells, green arrows indicate stromal cells, and blue arrows indicate skeletal muscle cells. Original magnification, × 400.

Histologic assessment of wound healing and response to DEX. Paraformaldehyde-fixed and stained sections are shown from the skin of nonwounded mice and from mouse wounds isolated 7 days after wounding in female Vegfr2-luc mice treated with saline or DEX (n = 4 per group). Sections were taken vertically through the granulated tissue extending from the surface into the adipose and skeletal muscle. In the sections shown, the surface of the skin or granulated tissue is at the top of the image, and in some images the deeper adipose tissue and skeletal muscle are readily visible. (A) Blood vessels (red arrows) were visualized by staining with an anti-CD31 antibody. The granulation tissue of saline-treated wounds showed blood vessels that were of relatively small caliber. In DEX-treated wounds, the blood vessels in the granulated tissue were of larger caliber. Dermal blood vessels in the normal nonwounded skin tissue were substantially less dense. Original magnification, × 200. (B) Infiltration of leukocytes was visualized by staining with anti-CD11b antibody. There were few scattered leukocytes (red arrows) in normal nonwounded skin. Blue arrow points to hair follicles in normal skin. Saline-treated wounds had significant leukocyte infiltration that appeared to consist predominantly of macrophages. DEX-treated wounds had markedly less macrophage infiltration. Original magnification, × 200. (C) The presence of the VEGFR2 receptor in the tissue was assessed by immunohistochemical staining with an anti-VEGFR2 antibody. Compared with normal nonwounded skin tissue, saline-treated wounds showed increased staining in endothelial cells and in stromal cells. Staining intensity was higher in cells closest to the lesioned epithelium of the wound, with less staining in deeper tissue. Red arrows indicate blood vessels. DEX-treated wounds had less VEGFR2 staining. Original magnification, × 100. (D) At a higher magnification, the specific cell types that positively stained for VEGFR2 were more apparent. Red arrows indicate blood vessel endothelial cells, green arrows indicate stromal cells, and blue arrows indicate skeletal muscle cells. Original magnification, × 400.

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