Generation of TSLP transgenic mice and expression of transgene specific mRNA. (A) Schematic representation of the TSLP transgene construct containing the composite promoter CAGGS,²⁴  murine TSLP cDNA (TSLP), 2 epitope tags: V5 and polyhistidine (poly-His), and the rabbit β-globin polyadenylation signal (poly A). (B) RT-PCR analysis of TSLP transgene mRNA expression in bone marrow (BM), spleen (S), thymus (T), kidney (K), liver (L), and heart (H) was examined. TG indicates transgenic; HPRT, hypoxanthine phosphoribosyl-transferase.