In vivo selection of GFP⁺ peripheral blood cells in monkey RQ2258 following 2 treatment courses with TMTX and NBMPR-P. (A) Changes in peripheral blood counts associated with 2 treatment courses. The graph shows changes in the absolute neutrophil count (▦) and the platelet count (•) associated with a first cycle of TMTX 6 mg/kg/d × 5 and NBMPR-P 3 mg/kg/d × 5, and a second treatment course with peg-SCF and G-CSF given 8 days concurrently with TMTX 6 mg/kg/d × 5 and NBMPR-P 3 mg/kg/d × 5. Baseline values are shown as discontinuous symbols, with continuous lines representing posttreatment values. Note that the left y-axis indicates neutrophil counts in cells per microliter and the right y-axis indicates platelet counts in thousands of cells per microliter. (B) Percentage of GFP-expressing peripheral blood cells prior to and following 2 treatment courses. The bar graph shows values for granulocytes, monocytes, and lymphocytes prior to and following the 2 treatment courses. Note the maximum value of the y-axis is 16% and that the 2 treatment courses are indicated by brackets on the x-axis.