Colocalization of CD26 and CD45RA outside lipid rafts after cross-linking of CD26 in CTBCs. For staining CD45RO⁺ PBTCs, cells were incubated with biotinylated anti-CD26 mAb (1F7) and cross-linked with Texas red-conjugate streptavidin for patching, as described in “Materials and methods.” After fixation, cells were stained with anti-CD45RO-FITC (UCHL-1). For analysis of CD45RA and CD26, CD45RA⁺ PBTCs and CBTCs were incubated with biotinylated anti-CD26 mAb (1F7) and cross-linked with Texas red-conjugate streptavidin, as described in “Materials and methods.” Cells were fixed and stained with FITC-conjugated anti-CD45RA mAb (2H4) (A) and CTB-FITC (B) and visualized by confocal microscopy (original magnification, × 400).