Binding of hD1D2 to collagen and inhibition by scFv 10B12. (A) Binding of hD1D2 to collagen I fibers (•) or GPP10 (○). Mean absorbance ± SD is shown for triplicate wells in a single experiment, representative of at least 3 independent experiments. (B) Binding of hD1D2 (10 μg/mL) to collagen fibers, in the presence of scFv 10B12 (•) or 2D4 (anti-HLA-A2; ○). The binding in the presence of scFv is shown as a percentage of the signal obtained in the absence of scFv. Mean ± SD is shown for triplicate wells in a single experiment, representative of at least 3 independent experiments.