Platelet aggregation induced by collagen and CRP-XL is selectively inhibited by scFv 10B12. (A) Aggregation in response to increasing doses of collagen I fibers. (B) Effect of scFvs on response to 4 μg/mL collagen I fibers or (C) 10 μg/mL collagen I fibers. (D) Aggregation in response to increasing doses of CRP-XL. (E) Effects of scFvs on response to 4 μg/mL CRP-XL. (B,C,E) scFvs are shown by 10B12 (circles), 2D4 (anti-HLA A2; triangles), and 1C3 (squares, in panels B and E only). (F) Effect of scFv 10B12 on response to moderate doses of various agonists; collagen I fibers 1 μg/mL, ADP 5 μM, U46619 5 μM, EPI 4 μM, TRAP 10 μM. (A-E) The maximal rate of aggregation or “slope” is shown in open symbols, whereas the extent of aggregation is shown in closed symbols. (B,C,E,F) The response with scFv 10B12 is shown as a percentage of the response without scFv. All points or bars represent the mean ± SEM of measurements from at least 3 separate donors.