Binding of scFvs 10B12 and 1C3 to hD1D2 and hD1D2 mutants by ELISA. (A) Binding of 10B12 to hD1D2 (▪), hD1D2-117P (□), hD1D2-166S (○), hD1D2-59E (•), and triple mutant (▾). (B) Binding of 1C3 to the antigens as represented in panel A. Points represent mean ± SD of triplicate wells in 1 experiment, representative of 2 identical experiments.