Figure 1. Identification of Lin – c-kit... | American Society of Hematology

Figure 1.

$Figure 1. Identification of Lin–c-kit+IL-7R+ cells in the fetal circulation at various gestational ages. (A) FB cells from 10.0- to 15-dpc fetuses were 3-color stained with anti-Lin (TER, Gr-1, Thy-1, B220, CD19), anti–c-kit, and anti–IL-7R. The FACS profiles of c-kit versus IL-7R on Lin– cells are displayed in the top lane. FL cells from 11- to 15-dpc fetuses and cells from 10.0- to 11-dpc AGM region were also examined. The histograms shown in the left part of the figures indicate Lin expression profiles of 10.5-dpc FB, 11-dpc FL, and 10.0 AGM cells, and gates for Lin– fractions. The numbers in the panels represent the percentage of Lin– cells in each area indicated by a box. (B) Kinetics of the appearance of Lin–c-kit+ (c-kit+) cells and Lin–c-kit+IL-7R+ (IL-7R+) cells in FB and FL at various gestational ages are shown. Numbers of whole FB and FL cells per fetus are also plotted. (C) FB cells from 12-, 14-, and 15-dpc fetuses from nude mice were 3-color stained with anti-Lin, anti–c-kit, and anti–IL-7R. The expression profiles of c-kit versus IL-7R on Lin– cells are displayed. (D) FT cells from 12-dpc fetuses were stained in 3 colors with anti-Lin, anti–c-kit, and anti–IL-7R. The expression profile of c-kit versus IL-7R on Lin– cells is displayed.$

Identification of Lin^–c-kit⁺IL-7R⁺cells in the fetal circulation at various gestational ages. (A) FB cells from 10.0- to 15-dpc fetuses were 3-color stained with anti-Lin (TER, Gr-1, Thy-1, B220, CD19), anti–c-kit, and anti–IL-7R. The FACS profiles of c-kit versus IL-7R on Lin^– cells are displayed in the top lane. FL cells from 11- to 15-dpc fetuses and cells from 10.0- to 11-dpc AGM region were also examined. The histograms shown in the left part of the figures indicate Lin expression profiles of 10.5-dpc FB, 11-dpc FL, and 10.0 AGM cells, and gates for Lin^– fractions. The numbers in the panels represent the percentage of Lin^– cells in each area indicated by a box. (B) Kinetics of the appearance of Lin^–c-kit⁺ (c-kit⁺) cells and Lin^–c-kit⁺IL-7R⁺ (IL-7R⁺) cells in FB and FL at various gestational ages are shown. Numbers of whole FB and FL cells per fetus are also plotted. (C) FB cells from 12-, 14-, and 15-dpc fetuses from nude mice were 3-color stained with anti-Lin, anti–c-kit, and anti–IL-7R. The expression profiles of c-kit versus IL-7R on Lin^– cells are displayed. (D) FT cells from 12-dpc fetuses were stained in 3 colors with anti-Lin, anti–c-kit, and anti–IL-7R. The expression profile of c-kit versus IL-7R on Lin^– cells is displayed.

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