Presence of granzyme B and perforin in highly purified permeabilized PMNs as detected by cytofluorometry. (A) Saponin-permeabilized PMNs were incubated with an antibody to perforin or granzyme B. Next, a PE-conjugated antimouse IgG was added and after extensive washing an FITC-labelled antibody to CD666. On the top, the isotype controls are shown. (B) In another set of experiments, highly purified, saponin-permeabilized PMNs were incubated with antibodies to CD66b, perforin, or granzyme B; then, antimouse IgG-FITC was added (thick lines). The thin lines represent the isotype controls. (C) Expression of perforin and granzyme B was measured in 7 individuals; the data are summarized as box blots with the boxes containing 50% of the values. Horizontal bars represent the median; squares, the mean.