The Ad5f35GFP vector transduces PBMCs more efficiently than the Ad5GFP vector and is capable of inducing an Ad-specific T-cell immune response. (A) PBMCs were transduced using the Ad5f35GFP vector or the Ad5GFP vector at the MOIs indicated. The transduction efficiency was assessed based on percent GFP-positive cells. (B) Cells becoming transduced with the Ad5f35GFP vector were analyzed by isolating CD14⁺ cells, CD19⁺ cells, and CD3⁺ cells, transducing them with the Ad5f35GFP vector at MOI 200 and then assessing GFP expression after 24 hours. (C) PBMCs transduced with the Ad5f35GFP (▪) and Ad5GFP (▨) vectors were used to stimulate PBMCs in ELISPOT assay. Results are shown as SFCs per 10⁶ PBMCs. Error bars indicate the standard error of 3 experiments.