Ad-specific CTL lines are capable of subgroup and serotype cross-reactive recognition. An in vitro-reactivated CTL line from donor 2, generated using the Ad5f35 vector, is capable of recognizing and killing autologous fibroblast targets (▪) transduced with Ad2 (subgroup C), Ad4 (subgroup E), Ad5 (chimeric subgroups C and B), Ad7, and Ad11 (subgroup B) but not autologous fibroblasts alone or allogeneic targets infected with Ad5 and Ad11 at 3 effector-target (E/T) ratios: 20:1, 10:1, and 5:1.