Western blot analysis of HFE protein expression levels. Western blot analysis of HFE protein expression levels in isolated rat hepatocytes (lane 1) and Kupffer cells (lane 2). Whole rat liver (lane 3), mouse liver (lane 4), and mouse HFE gene–transfected HEK 293 cells (HEK293 m-HFE, lane 5) were included as controls. Lysates (100 μg) were loaded into each lane except for lane 5, where 30 μg HEK293 m-HFE lysate was loaded.