Role of hematopoietic cell TF expression in LPS-induced lethality. WT mice were irradiated and reconstituted with bone marrow from either low TF mice or littermate mTF^+/–/hTF⁺ control mice. (A) PCR analysis of DNA from peripheral blood cells from WT mice that underwent transplantation with bone marrow from either mTF^+/–/hTF⁺ or low TF mice was used to demonstrate reconstitution of the donor mice with recipient bone marrow 6 weeks after the transplantation. PCR was performed for the WT TF (WT) allele. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. First lanes contain molecular weight standards. (B) LPS-induced TAT complex levels (6 hours) (mean ± SE; n = 4to5per group). (C) Survival of endotoxemic mice (5 mg/kg LPS intraperitoneally) reconstituted with bone marrow from either mTF^+/–/hTF⁺ mice (solid line; n = 20) or low TF mice (dashed line; n = 22). For survival studies, we used mice that underwent transplantation with bone marrow derived from 3 independent donors for each genotype. (D) LPS-induced IL-6 expression in WT mice receiving bone marrow from mTF^+/–/hTF⁺ (□) or low TF (▪) mice (mean ± SE; n = 4 per group). Differences between the 2 groups were not statistically significant.