Analysis of HIV-1- and CMV-specific IL-2-secreting CD4 T cells in the blood and lymph nodes of progressors, LTNPs, and HIV-negative subjects. (A) Flow cytometry profiles of HIV-1- and CMV-specific IL-2-secreting CD4 T cells in blood of representative progressors and LTNPs. Blood mononuclear cells were stimulated with p55 gag and CMV lysates and stained with anti-CD4 PerCp Cy5.5, anti-CD69 FITC, and anti-IL-2 PE. The data show the expression of CD69 and IL-2 within CD4 T-cell populations. The cluster of events shown in red corresponds to the responder CD4 T cells, ie, coexpressing CD69 and IL-2, whereas the cluster of events in blue corresponds to the nonresponder CD4 T cells. The data are expressed as the percentage of cells coexpressing CD69 and IL-2 within CD4 T cells. (B) Mean ± SE of cumulative data on the percentage of HIV-1- and CMV-specific IL-2-secreting CD4 T cells in blood and lymph node of progressors, LTNPs, and HIV-negative subjects. At least 1.5 × 106 events were analyzed.
