Effects of CD44 ligation on the proliferation and cell cycle status of NB4 cells. (A) NB4 cells (10⁵/mL) were seeded in 96-well culture plates in complete culture medium containing anti-CD44 mAb A3D8 (2.5 μg/mL) and were cultured for 5 days at 37°C. Control cells were cultured with IgG1. Cell numbers and viability were evaluated by the trypan blue exclusion test. Data are means ± 1 SD calculated from 3 independent experiments using triplicate samples. This figure is from Charrad et al.¹⁹  (B) Cells were treated with IgG1 or A3D8 for 48 hours, and cell cycling was analyzed by flow cytometry (Multicycle program) after propidium iodide (PI) staining. G0/G1 represents Gap 0/Gap1 phase; S, DNA synthesis phase; and G 2/M, mitosis phase. Results represent 1 representative experiment of 3. Values are means ± 1 SD resulting from quadruplicate samples.