Hyaluronic acid inhibits KG1a cell growth and increases p27 level. (A) KG1a cells (105/mL) were seeded in 96-well culture plates in complete culture medium containing 100 μg to 500 μg/mL hyaluronic acid and were cultured for 3 days at 37°C. Cell numbers and viability were evaluated in triplicate by the trypan blue exclusion test. Data are means ± 1 SD calculated from 3 independent experiments using triplicate samples. (B) Western blot analysis of p27 in THP-1 cells treated with 500 μg hyaluronic acid for 0 hours and 24 hours. Protein levels were evaluated by densitometric scanning, corrected with respect to α-tubulin expression, and expressed relative to the value obtained in empty vector-infected cells at time 0 (arbitrarily 1). These relative protein level values are shown in italics below the lanes. Results represent 1 representative experiment of 3.
