American Society of Hematology

Figure 3.

$Figure 3. Association of IgM, Src-PTKs, and Csk with GEMs in BAL-17 cells. BAL-17 and CD45-deficient clone 12 cells were either left unstimulated (–) or stimulated (+) for 5 minutes at 37°C with 20 μg/mL (Fab′)2 fragments of antimouse IgM Ab, after which they were lysed in 0.5% Triton X-100 and subjected to sucrose gradient ultracentrifugation. GEMs (fractions 4 and 5) and heavy fractions (fractions 10-12) were separated by SDS-PAGE and blotted with Abs against Lyn, Fyn, Csk, and IgM. Control blots were performed with HRP-conjugated CTB subunit to detect the ganglioside GM1, or with Abs against paxillin and CD71. Numbers at the bottom are percentages of total molecules present in each fraction.$

Association of IgM, Src-PTKs, and Csk with GEMs in BAL-17 cells. BAL-17 and CD45-deficient clone 12 cells were either left unstimulated (–) or stimulated (+) for 5 minutes at 37°C with 20 μg/mL (Fab′)₂ fragments of antimouse IgM Ab, after which they were lysed in 0.5% Triton X-100 and subjected to sucrose gradient ultracentrifugation. GEMs (fractions 4 and 5) and heavy fractions (fractions 10-12) were separated by SDS-PAGE and blotted with Abs against Lyn, Fyn, Csk, and IgM. Control blots were performed with HRP-conjugated CTB subunit to detect the ganglioside GM1, or with Abs against paxillin and CD71. Numbers at the bottom are percentages of total molecules present in each fraction.

This Feature Is Available To Subscribers Only