American Society of Hematology

Figure 4.

$Figure 4. Dynamic association of CD45 with GEMs in B cells. WEHI-231 (A), BAL-17 (B), and splenic B (C) cells were left unstimulated or were stimulated with 20 μg/mL anti-IgM Ab for the times indicated. After lysis in 0.5% Triton X-100 and sucrose gradient ultracentrifugation, each fraction (A) or GEM fraction (fractions 4 and 5 combined) and total cell lysates (TCL; B-C), was separated by SDS-PAGE and blotted with anti-CD45 mAb and HRP-conjugated CTB subunit to detect GM-1. In the upper panel of panel C, GEM fractions and TCL were immunoprecipitated with anti-CD45 mAb and then subjected to blotting with anti-CD45 mAb. Right panels indicate the time courses of the percentages of total CD45 that was GEM-associated, as determined by densitometric analysis.$

Dynamic association of CD45 with GEMs in B cells. WEHI-231 (A), BAL-17 (B), and splenic B (C) cells were left unstimulated or were stimulated with 20 μg/mL anti-IgM Ab for the times indicated. After lysis in 0.5% Triton X-100 and sucrose gradient ultracentrifugation, each fraction (A) or GEM fraction (fractions 4 and 5 combined) and total cell lysates (TCL; B-C), was separated by SDS-PAGE and blotted with anti-CD45 mAb and HRP-conjugated CTB subunit to detect GM-1. In the upper panel of panel C, GEM fractions and TCL were immunoprecipitated with anti-CD45 mAb and then subjected to blotting with anti-CD45 mAb. Right panels indicate the time courses of the percentages of total CD45 that was GEM-associated, as determined by densitometric analysis.

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