American Society of Hematology

Figure 5.

$Figure 5. Inverse correlation between association of CD45 with GEMs and tyrosine phosphorylation of Lyn. WEHI-231 (•) and 10-5 (○) cells were stimulated with 20 μg/mL anti-IgM Ab for 0.5, 3, 15, 30, and 45 minutes, and then lysed in 0.5% Triton X-100. GEMs (fractions 4 and 5 combined) and TCL were subjected to SDS-PAGE and blotting with anti-CD45 mAb (A). Tyrosine phosphorylation of Lyn was examined by blotting with anti-PY mAb (B). Association of Lyn and IgM with GEMs was also examined by blotting with anti-Lyn and anti-IgM Abs (C, D). The panels on the right show percentages of GEM-associated CD45, Lyn, and IgM, and fold increases in tyrosine phosphorylation of Lyn. Fold increases of Lyn phosphorylation were calculated by setting the level of unstimulated WEHI-231 cells as 1. ip indicates immunoprecipitated.$

Inverse correlation between association of CD45 with GEMs and tyrosine phosphorylation of Lyn. WEHI-231 (•) and 10-5 (○) cells were stimulated with 20 μg/mL anti-IgM Ab for 0.5, 3, 15, 30, and 45 minutes, and then lysed in 0.5% Triton X-100. GEMs (fractions 4 and 5 combined) and TCL were subjected to SDS-PAGE and blotting with anti-CD45 mAb (A). Tyrosine phosphorylation of Lyn was examined by blotting with anti-PY mAb (B). Association of Lyn and IgM with GEMs was also examined by blotting with anti-Lyn and anti-IgM Abs (C, D). The panels on the right show percentages of GEM-associated CD45, Lyn, and IgM, and fold increases in tyrosine phosphorylation of Lyn. Fold increases of Lyn phosphorylation were calculated by setting the level of unstimulated WEHI-231 cells as 1. ip indicates immunoprecipitated.

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