American Society of Hematology

Figure 7.

$Figure 7. Negative regulation of GEM-associated Src-PTKs by CD45. WEHI-231 (A), BAL-17 (B), and splenic B (C) cells, and their CD45-deficient clones were left unstimulated (–) or were stimulated (+) for 5 minutes with 20 μg/mL anti-IgM Ab. Lyn in WEHI-231 and splenic B cells and Fyn in BAL-17 cells were then immunoprecipitated from GEMs (fractions 4 and 5) and subjected to IVK assays with enolase and Western blotting with Abs against Lyn and Fyn.$

Negative regulation of GEM-associated Src-PTKs by CD45. WEHI-231 (A), BAL-17 (B), and splenic B (C) cells, and their CD45-deficient clones were left unstimulated (–) or were stimulated (+) for 5 minutes with 20 μg/mL anti-IgM Ab. Lyn in WEHI-231 and splenic B cells and Fyn in BAL-17 cells were then immunoprecipitated from GEMs (fractions 4 and 5) and subjected to IVK assays with enolase and Western blotting with Abs against Lyn and Fyn.

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