Figure 1.
Identification of Cbl binding protein(s) in gastric juice. (A) Fractionation of protein bound [57Co]Cbl in the gastric juice by size exclusion chromatography on a Sephacryl S-200 column (Amersham Pharmacia, Piscataway, NJ). The ratio of Ve/Vo (elution volume/void volume) is plotted against protein bound radioactivity per fraction. The protein peaks marked HC and IF correspond, respectively, to the elution of haptocorrin and intrinsic factor from the column. (B) Immunoprecipitation of protein bound radioactivity with antiserum specific for IF and for HC.