Replication of HSV-1 in MyD88–/–, TLR9–/–, and control mice. (A-B, E) MyD88–/– and C57BL/6 control mice (8-week-old males; n = 6) were injected with 107 PFU KOS/Dlux/OriL HSV-1 in each hind footpad. (C-D, F) TLR9–/– and Balb/c controls (6- to 7-week-old males; n = 5) were injected with 2 × 106 PFU KOS/Dlux/OriL HSV-1 in each hind footpad. Viral replication and spatial distribution of replicating virus was visualized by bioluminescence imaging daily up to day 7. Representative images taken 24 hours and 6 days after infection are shown (A-D). Signal intensities from hind feet were measured as photon flux (photons/second) within defined regions of interest (E-F). Mean values ± SEM are shown for each time point. (G-H) C57BL/6J wild-type and MyD88–/– mice were infected with 2 × 106 PFU KOS HSV-1 in each eye after corneal scarification. Viral titers of eye swabs (G) and trigeminal ganglia (H) were measured by plaque assay at different time points. Logarithmic mean values ± SD are shown (n = 3).
