Figure 3.
Activation of ephrinB1 potentiates platelet aggregation. (A) Gel-filtered platelets (200 × 109/L) were incubated with either 2 μg/mL GST-EphB1, GST-ΔEphB1, GST-ephrinA4, or 20 μM ADP in presence of 100 μg/mL Alexa-Fluor 594–labeled fibrinogen as described in “Materials and methods.” The results shown are representative of 3 experiments. (B-D) Gel-filtered platelets (200 × 109/L to 400 × 109/L) were stimulated as indicated with U46619, SFFLRN, or ionomycin in the presence of GST-EphB1, GST-ΔEphB1, or buffer, in the order shown. Representative of 4 experiments.
