LPS increases hypoxic gene expression. Quiescent NR8383 cells were maintained under control conditions or in the presence of LPS (1 μg/mL) for 6 hours. Total RNA was extracted from cells and resolved on formaldehyde/agarose gels. Northern blot was performed using a specific radiolabeled cDNA probe for VEGF, PAI-1, GLUT1, and iNOS. An 18S rRNA probe was used as a control for gel loading.