Implication of the PKC and PI3K pathways in HIF-1α induction by LPS. NR8383 cells were pretreated or not for 15 minutes with Ly29004 (20 μM) or GF109203X (10 μM) and maintained under control conditions in the presence of 1 μg/mL LPS (A), 100 nM PMA (A), or under hypoxic conditions (B) for 6 hours. Total cell extracts (25 μg) were resolved by SDS-PAGE (8% gel) and immunoblotted using an anti-HIF-1α antiserum or an antiphospho-p70S6 kinase polyclonal antibody.