Expression of IL-15 and IP-10 in IFN-DCs from CML patients. DC cultures were generated by treating freshly isolated monocytes from CML patients with either IFN-α/GM-CSF or IL-4/GM-CSF for 3 days, followed by a further 20-hour incubation in the presence or absence of LPS. At that time point, total RNA was extracted from the different cell samples and RT-PCR was performed. PCR for IL-15, IP-10, and GAPDH was carried out as described in “Materials and methods.” The results obtained with cells from 1 representative patient out of 3 are shown.