Monocyte-derived DCs generated in the presence of IFN-α express high levels of bcl-2 and undergo apoptosis after LPS-induced stimulation. Three-day CML-DCs were generated as described in the legend to Figure 2 and either left untreated or treated with LPS for 20 hours. Total RNA was then extracted from the different cell samples and assayed for the expression of bcl-2 by RT-PCR. At the same time point, apoptosis was assayed by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL). Values are the mean ± SE of the results of 5 experiments performed with cells from different patients. The differences between CML-LPS/IFN-DCs and CML-LPS/IL-4-DCs were statistically significant (P < .05).