In vitro characterization of NF-κB1^-/- and wild-type macrophages. (A) Expression of macrophage differentiation markers was quantified by flow cytometry after staining for the indicated markers. (B) Secretion of TNF by wild-type and NF-κB1^-/- macrophages after stimulation with LPS (10 ng/mL) for the indicated times. (C) TNF expression of LPS (10 ng/mL)-stimulated wild-type and NF-κB1^-/- macrophages, assessed by qPCR. (D) Secretion of cytokines by wild-type and NF-κB1^-/- macrophages after LPS (10 ng/mL) stimulation for 6 hours (MCP1, IL-6, IL-12, IL10) or 24 hours (IFN-γ). Bars represent wild-type (□) and NF-κB1^-/- (▧) BM-derived macrophages. (A-B) Representative for 3 experiments. (C-D) Representative for 2 experiments. Error bars indicate SD.