Figure 3.
cDNA microarry analysis of the effects of antithrombin forms on the expression of select heparan sulfate proteoglycans in primary HUVECs. HUVECs were cultured with or without different antithrombin forms (20 μg/mL) in the presence and absence of VEGF (10 ng/mL) as indicated. Total mRNA was isolated from the cells, amplified, and analyzed by cDNA microarray as detailed in “Materials and methods.” The expression of the indicated heparan sulfate proteoglycan mRNAs relative to a universal RNA reference is shown for the different treatment conditions ± SEM.