Northern blotting analysis of perlecan mRNA expression in antithrombin-treated HUVECs. Total mRNA was isolated from HUVECs cultured under the conditions of Figure 4 and perlecan mRNA expression was analyzed by Northern blotting using a perlecan domain III cDNA probe as described in “Materials and methods.” The same blot was probed with GAPDH cDNA as a loading control. Normalized band intensities are shown.